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Immunohistochemical staining of human cerebral cortex shows distinct positivity in endothelial cells / blood vessels.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in endothelial cells / blood vessels.
Immunohistochemical staining of human cerebral cortex shows distinct positivity in endothelial cells / blood vessels.

Anti-SHISA7 Antibody

HPA058935
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSHISA7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SHISA7 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID729956
  • Target name
    SHISA7
  • Target description
    shisa family member 7
  • Target synonyms
    CKAMP59, protein shisa-7, GABA(A) receptor auxiliary subunit Shisa7, UPF0626 protein A, cystine-knot AMPAR modulating protein of 59 kDa, shisa homolog 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA6NL88
  • Protein Name
    Protein shisa-7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SHISA7 using anti-SHISA7 antibody (A18878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHISA7 antigen affinity purified polyclonal antibody (Catalog # A18878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SHISA7 at approximately 70KD. The expected band size for SHISA7 is at 70KD.
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