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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Anti-SIAE Antibody

HPA038052
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSIAE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SIAE Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54414
  • Target name
    SIAE
  • Target description
    sialic acid acetylesterase
  • Target synonyms
    AIS6, CSE-C, CSEC, LSE, YSG2, sialate O-acetylesterase, H-Lse, cytosolic sialic acid 9-O-acetylesterase homolog, sialic acid-specific acetylesterase II
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HAT2
  • Protein Name
    Sialate O-acetylesterase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SIAE using anti-SIAE antibody (A08046). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIAE antigen affinity purified polyclonal antibody (Catalog # A08046) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIAE at approximately 70 kDa. The expected band size for SIAE is at 58 kDa.
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