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Immunohistochemical staining of human skin shows strong cytoplasmic positivity in the basal layer of epidermal cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in the basal layer of epidermal cells.
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in the basal layer of epidermal cells.

Anti-SIRT3 Antibody

HPA026809
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSIRT3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SIRT3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23410
  • Target name
    SIRT3
  • Target description
    sirtuin 3
  • Target synonyms
    SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, NAD-dependent deacetylase sirtuin-3, mitochondrial, NAD-dependent protein delactylase sirtuin-3, SIR2-like protein 3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, regulatory protein SIR2 homolog 3, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin type 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NTG7
  • Protein Name
    NAD-dependent protein deacetylase sirtuin-3, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SIRT3 using anti-SIRT3 antibody (A01061-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 43 kDa. The expected band size for SIRT3 is at 43 kDa.
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Lane 1: Mouse Liver lysates; Lane 2: Mouse Kindey lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: MouseHeart lysates probed with SIRT3 Polyclonal Antibody, Unconjugated (bs-6105R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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