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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Anti-SIVA1 Antibody

HPA066693
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSIVA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SIVA1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10572
  • Target name
    SIVA1
  • Target description
    SIVA1 apoptosis inducing factor
  • Target synonyms
    apoptosis regulatory protein Siva; CD27-binding (Siva) protein; CD27BP; SIVA; Siva-1; Siva-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15304
  • Protein Name
    Apoptosis regulatory protein Siva
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SIVA/SIVA1 using anti-SIVA/SIVA1 antibody (A05419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIVA/SIVA1 antigen affinity purified polyclonal antibody (Catalog # A05419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIVA/SIVA1 at approximately 19 kDa. The expected band size for SIVA/SIVA1 is at 19 kDa.
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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
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ReactivityHuman
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