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Anti-SKAP2 Antibody

144-61181
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSKAP2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SKAP2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8935
  • Target name
    SKAP2
  • Target description
    src kinase associated phosphoprotein 2
  • Target synonyms
    PRAP, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, src kinase-associated phosphoprotein 2, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, SKAP-55HOM, SKAP55 homolog, retinoic acid-induced protein 70, src family-associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adapter protein with PH and SH3 domains
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75563
  • Protein Name
    Src kinase-associated phosphoprotein 2
  • Scientific Description
    SKAP2 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SKAP2 using anti-SKAP2 antibody (A06280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SKAP2 antigen affinity purified polyclonal antibody (Catalog # A06280-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SKAP2 at approximately 41 kDa. The expected band size for SKAP2 is at 41 kDa.
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