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Immunohistochemical staining of human lymph node shows moderate membranous positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows moderate membranous positivity in non-germinal center cells.
Immunohistochemical staining of human lymph node shows moderate membranous positivity in non-germinal center cells.

Anti-SLAMF6 Antibody

HPA051903
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLAMF6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLAMF6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID114836
  • Target name
    SLAMF6
  • Target description
    SLAM family member 6
  • Target synonyms
    activating NK receptor; CD352; KALI; KALIb; Ly108; natural killer-, T- and B-cell antigen; NK-T-B-antigen; NTBA; NTB-A; NTBA receptor; SF2000; SLAM family member 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DU3
  • Protein Name
    SLAM family member 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLAMF6 using anti-SLAMF6 antibody (A05310-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Ramos whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLAMF6 antigen affinity purified polyclonal antibody (Catalog # A05310-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLAMF6 at approximately 60 kDa. The expected band size for SLAMF6 is at 37 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, HL60 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, Mouse kidney tissue All lanes: SLAMF6 antibody at 5.1ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 38, 25 kDa Observed band size: 60 kDa
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