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Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.

Anti-SLC13A4 Antibody

HPA048582
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSLC13A4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC13A4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26266
  • Target name
    SLC13A4
  • Target description
    solute carrier family 13 member 4
  • Target synonyms
    Na(+)/sulfate cotransporter SUT-1; NAS2; solute carrier family 13 (sodium/sulfate symporter), member 4; solute carrier family 13 (sodium/sulfate symporters), member 4; solute carrier family 13 (sodium/sulphate symporters), member 4; solute carrier family 13 member 4; sulphate transporter 1; SUT1; SUT-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKG4
  • Protein Name
    Solute carrier family 13 member 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western blot All lanes: SLC13A4 antibody at 2ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 69 kDa Observed band size: 69 kDa
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Figure 1. Western blot analysis of SLC13A4 using anti-SLC13A4 antibody (A16132-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse testis tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC13A4 antigen affinity purified polyclonal antibody (Catalog # A16132-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC13A4 at approximately 62 kDa. The expected band size for SLC13A4 is at 69 kDa.
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