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Anti-SLC19A1 Antibody

144-62497
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSLC19A1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SLC19A1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID6573
  • Target name
    SLC19A1
  • Target description
    solute carrier family 19 member 1
  • Target synonyms
    CHMD, FOLT, IFC-1, IFC1, IMD114, MEGAF, REFC, RFC, RFC1, RFT-1, hRFC, hSLC19A1, reduced folate transporter, cyclic dinucleotide:anion antiporter SLC19A1, folate transporter 1, folate:anion antiporter SLC19A1, intestinal folate carrier 1, placental folate transporter, reduced folate carrier 1, reduced folate carrier protein, reduced folate transporter 1, solute carrier family 19 (folate transporter), member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41440
  • Protein Name
    Reduced folate transporter
  • Scientific Description
    SLC19A1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RFC/SLC19A1 using anti-RFC/SLC19A1 antibody (A01679-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RFC/SLC19A1 antigen affinity purified polyclonal antibody (Catalog # A01679-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RFC/SLC19A1 at approximately 65 kDa. The expected band size for RFC/SLC19A1 is at 65 kDa.
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