
Figure 1. Western blot analysis of SLC20A1 using anti-SLC20A1 antibody (A03537-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC20A1 antigen affinity purified polyclonal antibody (Catalog # A03537-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC20A1 at approximately 85 kDa. The expected band size for SLC20A1 is at 74 kDa.
Anti-SLC20A1 Antibody Picoband(r)
A03537-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSLC20A1
Overview
- SupplierBoster Bio
- Product NameAnti-SLC20A1 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID6574
- Target nameSLC20A1
- Target descriptionsolute carrier family 20 member 1
- Target synonymsgibbon ape leukemia virus receptor 1; GLVR1; Glvr-1; leukemia virus receptor 1 homolog; PIT1; PiT-1; sodium-dependent phosphate transporter 1; solute carrier family 20 (phosphate transporter), member 1
- HostRabbit
- IsotypeIgG
- Protein IDQ8WUM9
- Protein NameSodium-dependent phosphate transporter 1
- Scientific DescriptionBoster Bio Anti-SLC20A1 Antibody Picoband® catalog # A03537-1. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203