Figure 1. Western blot analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A51/52 antigen affinity purified polyclonal antibody (Catalog # A17353) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A51/52 at approximately 45 kDa. The expected band size for SLC25A51/52 is at 34 kDa.
Figure 1. Western blot analysis of SLC25A51/52 using anti-SLC25A51/52 antibody (A17353). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC25A51/52 antigen affinity purified polyclonal antibody (Catalog # A17353) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC25A51/52 at approximately 45 kDa. The expected band size for SLC25A51/52 is at 34 kDa.
Anti-SLC25A51/52 Antibody Picoband(r)
A17353-APC
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetSLC25A51
Overview
- SupplierBoster Bio
- Product NameAnti-SLC25A51/52 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateAPC (Allophycocyanin)
- Gene ID92014
- Target nameSLC25A51
- Target descriptionsolute carrier family 25 member 51
- Target synonymsCG7943, MCART1, mitochondrial nicotinamide adenine dinucleotide transporter SLC25A51, mitochondrial NAD(+) transporter SLC25A51, mitochondrial NAD+ transporter, mitochondrial carrier triple repeat 1, mitochondrial carrier triple repeat protein 1
- HostRabbit
- IsotypeIgG
- Protein IDQ3SY17
- Protein NameMitochondrial nicotinamide adenine dinucleotide transporter SLC25A52
- Scientific DescriptionBoster Bio Anti-SLC25A51/52 Antibody Picoband® catalog # A17353. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Monkey, Mouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203