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Immunohistochemical staining of human thyroid gland shows moderate positivity in apical membrane in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate positivity in apical membrane in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate positivity in apical membrane in glandular cells.

Anti-SLC26A4 Antibody

HPA042860
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLC26A4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC26A4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5172
  • Target name
    SLC26A4
  • Target description
    solute carrier family 26 member 4
  • Target synonyms
    DFNB4, EVA, PDS, TDH2B, pendrin, sodium-independent chloride/iodide transporter, solute carrier family 26 (anion exchanger), member 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43511
  • Protein Name
    Pendrin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC26A4 using anti-SLC26A4 antibody (A00919-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HK-2 whole cell lysates, Lane 2: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC26A4 antigen affinity purified polyclonal antibody (Catalog # A00919-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC26A4 at approximately 86KD. The expected band size for SLC26A4 is at 86KD.
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