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Immunohistochemical staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemical staining of human appendix shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-SLC2A10 Antibody

HPA041015
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSLC2A10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLC2A10
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81031
  • Target name
    SLC2A10
  • Target description
    solute carrier family 2 member 10
  • Target synonyms
    ATORS; ATS; glucose transporter type 10; GLUT10; GLUT-10; solute carrier family 2 (facilitated glucose transporter), member 10; solute carrier family 2, facilitated glucose transporter member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95528
  • Protein Name
    Solute carrier family 2, facilitated glucose transporter member 10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLUT10/SLC2A10 using anti-GLUT10/SLC2A10 antibody (A06461-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT10/SLC2A10 antigen affinity purified polyclonal antibody (Catalog # A06461-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT10/SLC2A10 at approximately 67KD. The expected band size for GLUT10/SLC2A10 is at 54KD.
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