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Anti-SLC9A2 (C-term) Antibody

102-21204
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSLC9A2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SLC9A2 (C-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB32092
  • Gene ID6549
  • Target name
    SLC9A2
  • Target description
    solute carrier family 9 member A2
  • Target synonyms
    NHE2, sodium/hydrogen exchanger 2, Na(+)/H(+) exchanger 2, solute carrier family 9 (sodium/hydrogen exchanger), member 2, solute carrier family 9, subfamily A (NHE2, cation proton antiporter 2), member 2
  • Host
    Rabbit
  • Protein IDQ9UBY0
  • Protein Name
    Sodium/hydrogen exchanger 2
  • Scientific Description
    Rabbit Anti-SLC9A2 (C-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC9A2 using anti-SLC9A2 antibody (PA2219). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC9A2 antigen affinity purified polyclonal antibody (Catalog # PA2219) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC9A2 at approximately 91KD. The expected band size for SLC9A2 is at 91KD.
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