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Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Anti-SLU7 Antibody

HPA035907
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSLU7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SLU7
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10569
  • Target name
    SLU7
  • Target description
    spliceosome associated SLU7
  • Target synonyms
    9G8, hSlu7, pre-mRNA-splicing factor SLU7, SLU7 homolog, splicing factor, SLU7 splicing factor homolog, splicing factor, step II splicing factor SLU7, zinc knuckle motif containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95391
  • Protein Name
    Pre-mRNA-splicing factor SLU7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SLU7 using anti-SLU7 antibody (A07557-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human T-47D whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLU7 antigen affinity purified polyclonal antibody (Catalog # A07557-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLU7 at approximately 68 kDa. The expected band size for SLU7 is at 68 kDa.
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