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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:2000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:2000 dilution.
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-Smad4 Rabbit Monoclonal Antibody (Clone RM277) at a 1:2000 dilution.

anti-Smad4 (human), Rabbit Monoclonal (RM277)

Research Use Only
REV-31-1158-00
RevMAb Biosciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMAD4
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-Smad4 (human), Rabbit Monoclonal (RM277)
  • Delivery Days Customer
    5
  • Antibody Specificity
    This antibody reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM277
  • Formulation
    Liquid
  • Gene ID4089
  • Target name
    SMAD4
  • Target description
    SMAD family member 4
  • Target synonyms
    deleted in pancreatic carcinoma locus 4; deletion target in pancreatic carcinoma 4; DPC4; JIP; MAD homolog 4; MADH4; mothers against decapentaplegic homolog 4; mothers against decapentaplegic, Drosophila, homolog of, 4; MYHRS; SMAD, mothers against DPP homolog 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13485
  • Protein Name
    Mothers against decapentaplegic homolog 4
  • Scientific Description
    Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor-beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads. - Recombinant Antibody. This antibody reacts to human Smad4. This antibody may also react to mouse or rat Smad4, as predicted by immunogen homology. Applications: WB, IHC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-beta signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Binding of the TGF-beta superfamily of ligands that includes Transforming Growth Factor-beta (TGF-beta) and bone morphogenetic protein (BMP) to its cognate receptor allows phosphorylation of Smad 1, 2, 3, 5 and 8 (R-Smad, Receptor Smad). This signals for heterotrimerization with Smad4 (co-Smad, co-mediator Smad) and translocation of the complex to the nucleus. Inhibitory or antagonistic Smad (I-Smad) that includes Smad 6 and 7, interact with activated R-Smads and attenuate the signaling pathway. Smad4 acts as a tumor suppressor protein by transcriptionally regulating its target genes such as Cyclin D1 (downregulation) and collagen (upregulation) that inhibit cell proliferation. Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203