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ProductsBack/Anti-SMARCE1 [RAB-S17]

Anti-SMARCE1 [RAB-S17]

AB01881-1.1-BT
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetSMARCE1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SMARCE1 [RAB-S17]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IP-MS; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-S17
  • Gene ID6605
  • Target name
    SMARCE1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit E1
  • Target synonyms
    BAF57, CSS5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1, chromatin remodeling complex BRG1-associated factor 57
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ969G3
  • Protein Name
    SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BAF57/SMARCE1 using anti-BAF57/SMARCE1 antibody (A03739-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAF57/SMARCE1 antigen affinity purified polyclonal antibody (Catalog # A03739-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAF57/SMARCE1 at approximately 54 kDa. The expected band size for BAF57/SMARCE1 is at 47 kDa.
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