Figure 3. Flow Cytometry analysis of HL-60 cells using anti-SMC4 antibody (A04887-1). Overlay histogram showing HL-60 cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC4 Antibody (A04887-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 3. Flow Cytometry analysis of HL-60 cells using anti-SMC4 antibody (A04887-1). Overlay histogram showing HL-60 cells stained with A04887-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SMC4 Antibody (A04887-1, 1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Anti-SMC4 Antibody Picoband(r)
A04887-1-HRP
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSMC4
Overview
- SupplierBoster Bio
- Product NameAnti-SMC4 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID10051
- Target nameSMC4
- Target descriptionstructural maintenance of chromosomes 4
- Target synonymsCAPC; CAP-C; chromosome-associated polypeptide C; SMC protein 4; SMC-4; SMC4 structural maintenance of chromosomes 4-like 1; SMC4L1; structural maintenance of chromosomes protein 4
- HostRabbit
- IsotypeIgG
- Protein IDQ9NTJ3
- Protein NameStructural maintenance of chromosomes protein 4
- Scientific DescriptionBoster Bio Anti-SMC4 Antibody Picoband® catalog # A04887-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203