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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-SMCR8 Antibody

HPA024646
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMCR8
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMCR8 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID140775
  • Target name
    SMCR8
  • Target description
    SMCR8-C9orf72 complex subunit
  • Target synonyms
    DENND8A, guanine nucleotide exchange protein SMCR8, Smith-Magenis syndrome chromosome region, candidate 8, smith-Magenis syndrome chromosomal region candidate gene 8 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TEV9
  • Protein Name
    Guanine nucleotide exchange protein SMCR8
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMCR8 using anti-SMCR8 antibody (A14158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMCR8 antigen affinity purified polyclonal antibody (Catalog # A14158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMCR8 at approximately 140 kDa. The expected band size for SMCR8 is at 105 kDa.
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