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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-SMG9 Antibody

HPA042096
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMG9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMG9 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56006
  • Target name
    SMG9
  • Target description
    SMG9 nonsense mediated mRNA decay factor
  • Target synonyms
    C19orf61, F17127_1, HBMS, NEDITPO, nonsense-mediated mRNA decay factor SMG9, protein smg-9 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H0W8
  • Protein Name
    Nonsense-mediated mRNA decay factor SMG9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C19orf61/SMG9 using anti-C19orf61/SMG9 antibody (A10233-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf61/SMG9 antigen affinity purified polyclonal antibody (Catalog # A10233-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf61/SMG9 at approximately 58 kDa. The expected band size for C19orf61/SMG9 is at 57-65 kDa.
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TargetSMG9
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Protein IDQ9H0W8
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Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Product group Antibodies
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ReactivityHuman
TargetSMG9
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