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Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells.
Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells.
Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells.

Anti-SMS Antibody

HPA029852
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSMS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SMS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6611
  • Target name
    SMS
  • Target description
    spermine synthase
  • Target synonyms
    MRSR, MRXSSR, SPMSY, SRS, SpS, spermine synthase, spermidine aminopropyltransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52788
  • Protein Name
    Spermine synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Spermine Synthase/SMS using anti-Spermine Synthase/SMS antibody (A01831-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermine Synthase/SMS antigen affinity purified polyclonal antibody (Catalog # A01831-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermine Synthase/SMS at approximately 41 kDa. The expected band size for Spermine Synthase/SMS is at 41 kDa.
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