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Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.
Immunohistochemical staining of human pancreas shows moderate nuclear positivity in exocrine glandular cells.

Anti-SNRPA1 Antibody

HPA048499
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetSNRPA1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNRPA1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6627
  • Target name
    SNRPA1
  • Target description
    small nuclear ribonucleoprotein polypeptide A'
  • Target synonyms
    Lea1; U2 small nuclear ribonucleoprotein A'; U2 small nuclear ribonucleoprotein polypeptide A'; U2 snRNP A'; U2A'
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09661
  • Protein Name
    U2 small nuclear ribonucleoprotein A'
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNRPA1 using anti-SNRPA1 antibody (A10655-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNRPA1 antigen affinity purified polyclonal antibody (Catalog # A10655-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNRPA1 at approximately 28 kDa. The expected band size for SNRPA1 is at 28 kDa.
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