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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Anti-SNRPC Antibody

HPA042419
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSNRPC
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNRPC
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6631
  • Target name
    SNRPC
  • Target description
    small nuclear ribonucleoprotein polypeptide C
  • Target synonyms
    U1 small nuclear ribonucleoprotein C; U1 small nuclear RNP specific C; U1 snRNP C; U1 snRNP protein C; U1C; Yhc1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U1-C/SNRPC using anti-U1-C/SNRPC antibody (A07728). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1-C/SNRPC antigen affinity purified polyclonal antibody (Catalog # A07728) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1-C/SNRPC at approximately 17 kDa. The expected band size for U1-C/SNRPC is at 17 kDa.
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