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Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.
Immunofluorescent staining of human cell line JURKAT shows localization to plasma membrane.

Anti-SNTG2 Antibody

HPA065258
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSNTG2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNTG2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54221
  • Target name
    SNTG2
  • Target description
    syntrophin gamma 2
  • Target synonyms
    G2SYN, SYN5, gamma-2-syntrophin, syntrophin-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NY99
  • Protein Name
    Gamma-2-syntrophin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SNTG2 using anti-SNTG2 antibody (A11619-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNTG2 antigen affinity purified polyclonal antibody (Catalog # A11619-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNTG2 at approximately 60 kDa. The expected band size for SNTG2 is at 60 kDa.
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Immunohistochemical staining of human hippocampus shows strong positivity in glial cells.
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