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Immunohistochemical staining of human kidney shows moderate cytoplasmic and nucleolar positivity in tubular cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nucleolar positivity in tubular cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and nucleolar positivity in tubular cells.

Anti-SNX15 Antibody

HPA038955
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSNX15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNX15
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29907
  • Target name
    SNX15
  • Target description
    sorting nexin 15
  • Target synonyms
    clone iota unknown protein; HSAF001435; sorting nexin-15
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NRS6
  • Protein Name
    Sorting nexin-15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA048443(SNX15 Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of SNX15 using anti-SNX15 antibody (A11463-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX15 antigen affinity purified polyclonal antibody (Catalog # A11463-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX15 at approximately 50 kDa. The expected band size for SNX15 is at 38 kDa.
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