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Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Anti-SNX4 Antibody

HPA005709
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSNX4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SNX4
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8723
  • Target name
    SNX4
  • Target description
    sorting nexin 4
  • Target synonyms
    ATG24B; sorting nexin-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95219
  • Protein Name
    Sorting nexin-4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNX4 using anti-SNX4 antibody (A08783-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX4 antigen affinity purified polyclonal antibody (Catalog # A08783-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX4 at approximately 55 kDa. The expected band size for SNX4 is at 52 kDa.
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