Anti-Sonic Hedgehog [5E1]

Absolute Antibody

Anti-Sonic Hedgehog [5E1]

9

This antibody is specific for the amino-terminal domain of rat SHH (SHH-N). It cross-reacts with the two other mammalian hedgehog ligands, Indian Hedgehog and Desert Hedgehog.

The binding specificity of this antibody for the amino-terminal fragment of SHH (SHH-N) has been confirmed in ELISA and Western blot analysis (Ericson et al, 1996).This antibody has been used to detect SHH in IHC analysis (Ericson et al, 1996).This antibody is able to inhibit hedgehog signalling, as it blocks the binding of all three mammalian Hh ligands to Ptc1 with low nanomolar affinity (Bosanac et al, 2009). In vivo, this inhibition is responsible for the observed growth retardation of HT55 an HT-29 xenografts in mice tumour models (Yauch et al, 2008). A Fab fragment derived from this antibody has been used in X ray crystallography analysis, in complex with human SHH (Maun, 2010).

Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

5E1

Shh

sonic hedgehog signaling molecule

shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC; sonic hedgehog homolog; sonic hedgehog protein

Rabbit

IgG

Sonic hedgehog protein

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human, Rat

-20°C,2°C to 8°C

12352203