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Figure 1. Western blotting validation for Anti-Sorting nexin-5 SNX5 Antibody A04788 Western blot analysis of extracts of various cell lines
Figure 1. Western blotting validation for Anti-Sorting nexin-5 SNX5 Antibody A04788 Western blot analysis of extracts of various cell lines
Figure 1. Western blotting validation for Anti-Sorting nexin-5 SNX5 Antibody A04788 Western blot analysis of extracts of various cell lines

Anti-Sorting nexin-5 SNX5 Antibody

A04788
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSNX5
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Sorting nexin-5 SNX5 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID27131
  • Target name
    SNX5
  • Target description
    sorting nexin 5
  • Target synonyms
    sorting nexin-5
  • Host
    Rabbit
  • Protein IDQ9Y5X3
  • Protein Name
    Sorting nexin-5
  • Scientific Description
    Boster Bio Anti-Sorting nexin-5 SNX5 Antibody catalog # A04788. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SNX5 using anti-SNX5 antibody (A04788-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SNX5 antigen affinity purified polyclonal antibody (Catalog # A04788-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SNX5 at approximately 47 kDa. The expected band size for SNX5 is at 47 kDa.
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