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Immunohistochemical staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear immunoreactivity in a subset of glial cells.

Anti-SOX10 Antibody

AMAB91297
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSOX10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOX10
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL4455
  • Conjugate
    Unconjugated
  • Gene ID6663
  • Target name
    SOX10
  • Target description
    SRY-box transcription factor 10
  • Target synonyms
    DOM, PCWH, SOX-10, WS2E, WS4, WS4C, transcription factor SOX-10, SRY (sex determining region Y)-box 10, SRY-box 10, SRY-related HMG-box gene 10, dominant megacolon, mouse, human homolog of
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP56693
  • Protein Name
    Transcription factor SOX-10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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IHC image of CSB-PA022418OA01HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA068898 antibody. Corresponding SOX10 RNA-seq data are presented for the same tissues.
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Figure 1. Western blot analysis of SOX10 using anti-SOX10 antibody (A00758-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human U87 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX10 antigen affinity purified polyclonal antibody (Catalog # A00758-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX10 at approximately 60 kDa. The expected band size for SOX10 is at 50 kDa.
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