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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

Anti-SOX2-25ul

HPA045725
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOX2-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Applications Supplier
    WB, ChIP, ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48431
  • Protein Name
    Transcription factor SOX-2
  • Scientific Description
    Rabbit Polyclonal Anti-SOX2 Antibody against Human SRY (sex determining region Y)-box 2. Validated for Chromatin Immunoprecipitation, Immunofluorescence and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOX2 using anti-SOX2 antibody (A00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 ma for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified poly clonal antibody (Catalog # A00105-1) at 0.5 microg/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit ING-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Luminescence detection (ECL) kit (Catalog # EK1002) with Anon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
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