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Immunohistochemical staining of human Placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human Placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human Placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.

Anti-SOX7 Antibody

HPA009065
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSOX7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SOX7 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83595
  • Target name
    SOX7
  • Target description
    SRY-box transcription factor 7
  • Target synonyms
    transcription factor SOX-7, SRY (sex determining region Y)-box 7, SRY-box 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BT81
  • Protein Name
    Transcription factor SOX-7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SOX7 using anti-SOX7 antibody (A05013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Colo320 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX7 antigen affinity purified polyclonal antibody (Catalog # A05013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX7 at approximately 43 kDa. The expected band size for SOX7 is at 43 kDa.
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