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Anti-SP5 (C-term) Antibody

102-27838
RayBiotech
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSP5
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SP5 (C-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB15102
  • Gene ID389058
  • Target name
    SP5
  • Target description
    Sp5 transcription factor
  • Target synonyms
    transcription factor Sp5
  • Host
    Rabbit
  • Protein IDQ6BEB4
  • Protein Name
    Transcription factor Sp5
  • Scientific Description
    Rabbit Anti-SP5 (C-term) Antibody, 400 microl
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SP5 using anti-SP5 antibody (PB9443). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SP5 antigen affinity purified polyclonal antibody (Catalog # PB9443) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SP5 at approximately 42 kDa. The expected band size for SP5 is at 42 kDa.
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