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Immunohistochemical staining of human prostate shows no positivity in glandular cell as expected.
Immunohistochemical staining of human prostate shows no positivity in glandular cell as expected.
Immunohistochemical staining of human prostate shows no positivity in glandular cell as expected.

Anti-SPACA3 Antibody

HPA023633
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPACA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPACA3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID124912
  • Target name
    SPACA3
  • Target description
    sperm acrosome associated 3
  • Target synonyms
    ALLP17; cancer/testis antigen 54; CT54; LYC3; lysozyme C; lysozyme-like acrosomal sperm-specific secretory protein ALLP-17; lysozyme-like protein 3; lysozyme-like sperm-specific secretory protein ALLP17; LYZC; LYZL3; SLLP1; sperm acrosome membrane-associated protein 3; sperm lysozyme-like protein 1; sperm lyzozyme-like acrosomal protein 1; sperm protein reactive with antisperm antibodies; sperm protein reactive with ASA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IXA5
  • Protein Name
    Sperm acrosome membrane-associated protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of SPACA3 using anti-SPACA3 antibody (A10284-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPACA3 antigen affinity purified polyclonal antibody (Catalog # A10284-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPACA3 at approximately 40 kDa. The expected band size for SPACA3 is at 23 kDa.
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