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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA040446 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA040446 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA040446 antibody. Corresponding SPAG9 RNA-seq data are presented for the same tissues.

Anti-SPAG9 Antibody

HPA040446
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSPAG9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPAG9
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9043
  • Target name
    SPAG9
  • Target description
    sperm associated antigen 9
  • Target synonyms
    cancer/testis antigen 89; c-Jun NH2-terminal kinase-associated leucine zipper protein; C-Jun-amino-terminal kinase-interacting protein 4; CT89; HLC4; HLC6; HLC-6; human lung cancer oncogene 6 protein; JIP4; JIP-4; JLP; JNK interacting protein; JNK/SAPK-associated protein; lung cancer oncogene 4; Max-binding protein; mitogen-activated protein kinase 8-interacting protein 4; PHET; PIG6; proliferation-inducing gene 6; protein highly expressed in testis; sperm associated antigen 9 variant 5; sperm surface protein; sunday driver 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60271
  • Protein Name
    C-Jun-amino-terminal kinase-interacting protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of JLP/SPAG9 using anti-JLP/SPAG9 antibody (A02751-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JLP/SPAG9 antigen affinity purified polyclonal antibody (Catalog # A02751-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JLP/SPAG9 at approximately 190 kDa. The expected band size for JLP/SPAG9 is at 190 kDa.
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