Figure 1. IF analysis of SPAK/STK39 using anti-SPAK/STK39 antibody (A02516-3). SPAK/STK39 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5microg/mL rabbit anti-SPAK/STK39 Antibody (A02516-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Figure 1. IF analysis of SPAK/STK39 using anti-SPAK/STK39 antibody (A02516-3). SPAK/STK39 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5microg/mL rabbit anti-SPAK/STK39 Antibody (A02516-3) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Anti-SPAK/STK39 Antibody Picoband(r)
A02516-3-CY3
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTK39
Overview
- SupplierBoster Bio
- Product NameAnti-SPAK/STK39 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID27347
- Target nameSTK39
- Target descriptionserine/threonine kinase 39
- Target synonymsDCHT; PASK; proline-alanine-rich STE20-related kinase; serine threonine kinase 39 (STE20/SPS1 homolog, yeast); serine/threonine-protein kinase 39; small intestine SPAK-like kinase; SPAK; STE20/SPS1 homolog; STE20/SPS1-related proline-alanine-rich protein kinase; Ste20-like protein kinase; ste-20-related kinase
- HostRabbit
- IsotypeIgG
- Protein IDQ9UEW8
- Protein NameSTE20/SPS1-related proline-alanine-rich protein kinase
- Scientific DescriptionBoster Bio Anti-SPAK/STK39 Antibody Picoband® catalog # A02516-3. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203