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Anti-SPDEF Antibody

144-66214
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSPDEF
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SPDEF Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID25803
  • Target name
    SPDEF
  • Target description
    SAM pointed domain containing ETS transcription factor
  • Target synonyms
    PDEF, bA375E1.3, SAM pointed domain-containing Ets transcription factor, prostate epithelium-specific Ets transcription factor, prostate-derived Ets factor, prostate-specific Ets
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95238
  • Protein Name
    SAM pointed domain-containing Ets transcription factor
  • Scientific Description
    SPDEF pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSE/SPDEF using anti-PSE/SPDEF antibody (A04625-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSE/SPDEF antigen affinity purified polyclonal antibody (Catalog # A04625-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSE/SPDEF at approximately 44 kDa. The expected band size for PSE/SPDEF is at 38 kDa.
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A549 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PDEF/SPDEF Polyclonal Antibody(bs-1866R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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