Anti-Spike protein [4A8]

Absolute Antibody

Anti-Spike protein [4A8]

7

The binding profile of this antibody was determined by performing an ELISA on SARS-CoV-2 S, S1, S2 or RBD protein using the human version of this antibody. Furthermore a neutralization experiment on Vero-E6 cells infected with SARS CoV2 was done using the human IgG1 version of this antibody. The binding of the human version was also characterized using biolayer interferometry. A flow cytometric receptor binding inhibition assay was also performed on the human version of this antibody incubated in SARS-CoV-2S protein and then incubated with ACE2-293T cells. The complex of S-ECD and the human version of this antibody was characterized using cryo electromicroscopy (Chi et al, 2020; pmid:32571838).

Functional Assay, Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

4A8

S

surface glycoprotein

GU280_gp02, spike glycoprotein, surface glycoprotein

Human

IgG1

Spike glycoprotein

-20°C,2°C to 8°C

41116161