Anti-Spike protein [MR17 ]

Absolute Antibody

Anti-Spike protein [MR17 ]

7

Wild type MR17 had a modest neutralization activity. ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). IC50 value of wild type MR17 was determined to be 12.32 microg m/L. This sybody is a K99Y mutant version of wild typr MR17. It is seen that the single mutation increased the binding affinity by 1.5 fold and decreased the off-rate by 2.8 fold. Overall neutralization activity was increased 24 fold after the mutation was introduced, with an IC50 value of 0.50 microg m/L. The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203