Anti-Spike protein [MR3]

Absolute Antibody

Anti-Spike protein [MR3]

7

MR3 is a potent antibody that binds the RBD with an affiinty of KD=1.0 nM and neutralizes SARS-CoV-2 pseudovirus with an IC50 of 0.40 microg m/L. Consistent with its highest affinity, MR3 showed the slowest off-rate (2.3 x 10-4 per second). ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203