Anti-spike protein [PZZ 21]

Absolute Antibody

Anti-spike protein [PZZ 21]

7

The specificity of the original format of the antibody was confirmed by ELISA analysis. Immunofluorescence assay revealed that the scFv stained PEDV-infected Vero E6 cells but did not react with uninfected control cells. The scFv was shown to inhibit PEDV infectivity by the plaque reduction neutralization assay (the virus neutralization titer was 6.25 microg/mL). Results showed the a cocktail of the scF fragment, PZZ 24 and PZZ 35 neutralized viral infection at 3.125 microg/mL. Finally, piglets orally administered with a mixture of scFv fragment, PZZ 24 and PZZ 35 showed no to mild clinical symptoms, significantly less viral shedding, no mortality and no intestinal lesions.

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original scFv format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203