Bio-Connect

Anti-Spike protein [SR4]

Ab02058-23.159
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Neutralisation/Blocking, Other Application
Product group Antibodies
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Spike protein [SR4]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Structural alignment of the SR4-RBD complex and ACE2-RBD complex revealed that binding of SR4 did not cause any noticable conformation changes of the RBD. ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). IC50 value of SR4 was determined to be 5.90 microg m/L. The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).
  • Applications
    Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203