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Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.
Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.
Western blot analysis of different cell lysates, cytosol or nuclear enriched fractions, using mouse mAb to splicing factor SF3B4, M06414, dilution 1:1,000 in green: [1] protein standard (red), [2] NIH-3T3 cytosolic fraction [3] NIH-3T3 nuclear fraction [4] HeLa cytosolic and [5] HeLa nuclear fractions. Strong single band at 49kDa represents the SF3B4 protein, which is expressed exclusively in the nuclei. The same blot was simultaneously probed with rabbit pAb to GAPDH, dilution 1:20,000, in red. The 37kDa band corresponds to the GAPDH protein, detected mainly in the cytosolic fractions of these cells.

Anti-Splicing Factor SF3B4 Monoclonal Antibody

M06414
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSF3B4
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Splicing Factor SF3B4 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID10262
  • Target name
    SF3B4
  • Target description
    splicing factor 3b subunit 4
  • Target synonyms
    AFD1; Hsh49; pre-mRNA-splicing factor SF3b 49 kDa subunit; SAP 49; SAP49; SF3b49; SF3b50; spliceosomal protein; spliceosome-associated protein (U2 snRNP); spliceosome-associated protein 49; splicing factor 3B subunit 4; splicing factor 3b, subunit 4, 49kD; splicing factor 3b, subunit 4, 49kDa
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ15427
  • Protein Name
    Splicing factor 3B subunit 4
  • Scientific Description
    Boster Bio Anti-Splicing Factor SF3B4 Monoclonal Antibody catalog # M06414. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203