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Anti-SPOCK3 Antibody

144-62515
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSPOCK3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SPOCK3 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID50859
  • Target name
    SPOCK3
  • Target description
    SPARC (osteonectin), cwcv and kazal like domains proteoglycan 3
  • Target synonyms
    HSAJ1454, TES-3, TICN3, testican-3, SPARC/osteonectin, cwcv and kazal like domains proteoglycan 3, sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQ16
  • Protein Name
    Testican-3
  • Scientific Description
    SPOCK3 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPOCK3 using anti-SPOCK3 antibody (A03151-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOCK3 antigen affinity purified polyclonal antibody (Catalog # A03151-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOCK3 at approximately 57 kDa. The expected band size for SPOCK3 is at 57 kDa.
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