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Immunohistochemical staining of human prostate cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human prostate cancer shows strong cytoplasmic positivity in tumor cells.
Immunohistochemical staining of human prostate cancer shows strong cytoplasmic positivity in tumor cells.

Anti-SPON2 Antibody

HPA040170
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPON2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPON2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10417
  • Target name
    SPON2
  • Target description
    spondin 2
  • Target synonyms
    DIL-1, DIL1, M-SPONDIN, MINDIN, spondin-2, differentially expressed in cancerous and non-cancerous lung cells 1, spondin 2, extracellular matrix protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BUD6
  • Protein Name
    Spondin-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SPON2 using anti-SPON2 antibody (A07465-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human LNCAP whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPON2 antigen affinity purified polyclonal antibody (Catalog # A07465-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPON2 at approximately 39 kDa. The expected band size for SPON2 is at 36 kDa.
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