Anti-Sporozoite of Eimeria acervulina [6D-12-G10]

Absolute Antibody

Anti-Sporozoite of Eimeria acervulina [6D-12-G10]

9

This antibody recognizes and binds the apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa protein.

Avain coccidiosis is caused by an intracellulae protozoan belonging to the genus Eimeria. This disease is important in aviculture due to its negative effects on production and development of birds. Avian coccidiosis is one of the main causes of growth retardation, poor feed conversion and high mortality in intensive farming of broilers, cage-free pullets and breeders. This mAb recognizes the apical complex of the sporozoite in a western blot assay and ELISA. Immunoelectron microscopic examination revealed that the antibody stained the conoid antigen. In indirect immunofluorescence assay the culture supernatent containing the mAb intensely stained the tip of the E. acervulina sporozoites. It is also reported that this antibody inhibited the invasion of sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was produced at a minimum of 7 mg l-1 and exhibited virtually identical antigen reactivity as the original mAb (Min et al., 2001). mAb 6D-12-G10 shows cross reactivities in immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum (PMID:15725532). This mAb is also cross reactive with other species like Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp (PMID: 27384177)

ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

6D-12-G10

Mouse

IgG2b

This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.

Parasite

-20°C,2°C to 8°C

12352203