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Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal and glial cells.

Anti-SPRED2 Antibody

HPA064394
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPRED2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SPRED2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID200734
  • Target name
    SPRED2
  • Target description
    sprouty related EVH1 domain containing 2
  • Target synonyms
    NS14, Spred-2, sprouty-related, EVH1 domain-containing protein 2, sprouty protein with EVH-1 domain 2, related sequence
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z698
  • Protein Name
    Sprouty-related, EVH1 domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPRED2 using anti-SPRED2 antibody (A06368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPRED2 antigen affinity purified polyclonal antibody (Catalog # A06368-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPRED2 at approximately 53 kDa. The expected band size for SPRED2 is at 48 kDa.
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