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Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.

Anti-SRM Antibody

HPA015746
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSRM
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SRM
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6723
  • Target name
    SRM
  • Target description
    spermidine synthase
  • Target synonyms
    PAPT; putrescine aminopropyltransferase; SPDSY; spermidine synthase; spermidine synthase-1; SPS1; SRML1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19623
  • Protein Name
    Spermidine synthase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: SRM antibody at 2microg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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Figure 1. Western blot analysis of Spermidine synthase/SRM using anti-Spermidine synthase/SRM antibody (A01594-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Spermidine synthase/SRM antigen affinity purified polyclonal antibody (Catalog # A01594-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Spermidine synthase/SRM at approximately 34 kDa. The expected band size for Spermidine synthase/SRM is at 34 kDa.
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