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Immunohistochemical staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human gallbladder shows strong cytoplasmic positivity in glandular cells.

Anti-SRP54 Antibody

HPA048977
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSRP54
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SRP54
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6729
  • Target name
    SRP54
  • Target description
    signal recognition particle 54
  • Target synonyms
    SCN8; signal recognition particle 54 kDa protein; signal recognition particle 54kD; signal recognition particle 54kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61011
  • Protein Name
    Signal recognition particle 54 kDa protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SRP54 using anti-SRP54 antibody (A06189-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SRP54 antigen affinity purified polyclonal antibody (Catalog # A06189-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SRP54 at approximately 256 kDa. The expected band size for SRP54 is at 256 kDa.
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Immunofluorescent staining of human cell line Hep G2 shows localization to cytosol.
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