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Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.

Anti-STOML3 Antibody

HPA012892
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSTOML3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STOML3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID161003
  • Target name
    STOML3
  • Target description
    stomatin like 3
  • Target synonyms
    Epb7.2l, SRO, stomatin-like protein 3, EPB72-like 3, SLP-3, erythrocyte band 7 integral membrane protein, protein 7.2B, stomatin (EPB72)-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TAV4
  • Protein Name
    Stomatin-like protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of STOML3 using anti-STOML3 antibody (A13661-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat ovary tissue lysates, Lane 2: rat SHZ-88 whole cell lysates, Lane 3: mouse ovary tissue lysates, Lane 4: mouse ID8 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STOML3 antigen affinity purified polyclonal antibody (Catalog # A13661-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STOML3 at approximately 36 kDa. The expected band size for STOML3 is at 32 kDa.
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