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Anti-Stonin-2 [N346/9]

Ab02174-10.0
Absolute Antibody
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSTON2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Stonin-2 [N346/9]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for detection and analysis of stonin-2 by immunocytochemistry, immunohistochemistry and western blot. For instance, the mouse version of this antibody was used to detect stonin-2 by western blot in extracts of COS cells transiently transfected with HA-tagged Stonin-1, Stonin-2 or untagged Kv2.1 plasmid. Furthermore it was used to detect stonin-2 by immunocytochemistry in adult hippocampus from Stonin-2 wild-type and knockout mice.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; ICC; IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    N346/9
  • Gene ID85439
  • Target name
    STON2
  • Target description
    stonin 2
  • Target synonyms
    STN2, STNB, STNB2, stonin-2, stoned B homolog 2
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ8WXE9
  • Protein Name
    Stonin-2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    human, mouse, rat
  • Reactivity Supplier Note
    This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-555 (N-terminus) of human Stonin-2.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STON2 using anti-STON2 antibody (A10624). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STON2 antigen affinity purified polyclonal antibody (Catalog # A10624) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STON2 at approximately 130 kDa. The expected band size for STON2 is at 101 kDa.
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