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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic and strong nucleolar positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic and strong nucleolar positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic and strong nucleolar positivity in Purkinje cells.

Anti-STRAP Antibody

HPA027320
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTRAP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STRAP Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11171
  • Target name
    STRAP
  • Target description
    serine/threonine kinase receptor associated protein
  • Target synonyms
    MAWD, PT-WD, UNRIP, serine-threonine kinase receptor-associated protein, MAP activator with WD repeats, MAPK activator with WD repeats, WD-40 repeat protein PT-WD, mitogen-activated protein kinase activator with WD repeats, unr-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3F4
  • Protein Name
    Serine-threonine kinase receptor-associated protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Unrip using anti-Unrip antibody (PB9407). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates Lane 2: human Jurkat whole cell lysates Lane 3: human PC-3 whole cell lysates Lane 4: human THP-1 whole cell lysates Lane 5: rat brain tissue lysates Lane 6: mouse HEPA1-6 whole cell lysates Lane 7: mouse RAW246.7 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Unrip antigen affinity purified polyclonal antibody (Catalog # PB9407) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Unrip at approximately 39KD. The expected band size for Unrip is at 39KD.
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